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ZEISS ApoTome 3D Image Gallery

Imaging through thick tissue sections is hampered by blurring and stray light that occurs due to fluorescence emission arising from regions that are removed from the objective focal plane. In many cases, the effects of this high background signal can be so severe that finely labeled structures and critical image details are completely obscured. The ZEISS ApoTome employs structured illumination to generate optical sections of thick specimens that exhibit dramatically increased sharpness and contrast. These optical sections are useful to create three-dimensional (3D) reconstructions of the original sample. The digital images in this gallery are reconstructions of animal and plant tissue optical sections acquired with the ApoTome coupled to a metal halide light source.