mTFP1 Fused to Human EB3
Teal fluorescent proteins have spectral properties that are red-shifted from classical ECFP and blue-shifted with respect to EGFP. The highest performance member of this class is teal fluorescent protein (mTFP1). The localization of mTFP1 fused to human EB3 is demonstrated in the digital image appearing above. EB3 is a member of the family of end-binding proteins (known as EBs) that specifically associate with the ends of growing microtubules in a variety of cell types. Studies suggest that these proteins regulate microtubule dynamics and the binding of microtubules to organelles, membrane components, and other protein complexes.