mEmerald Fused to an ER Targeting Peptide
The interconnected network of sacs and tubules known as the endoplasmic reticulum (ER) functions in the manufacture, processing, and transportation of an array of biochemical compounds for use inside and outside of the cell. The ER was highlighted in the mammalian cell specimen appearing in the digital image above with monomeric Emerald fluorescent protein (mEmerald) fused to an endoplasmic reticulum targeting peptide. This fluorescent protein variant is derived from EGFP through the addition of several mutations that improve folding. The monomeric protein exhibits a peak excitation wavelength of 487 nanometers and a peak emission wavelength of 509 nanometers.