Contact Us | Carl Zeiss

Zeiss Logo

Education in Microscopy and Digital Imaging

ZEISS Microscopy ¦ Products ¦ Solutions ¦ Support ¦ Online Shop ¦ ZEISS International

Light Sources Spectral Imaging Wavelength Selection Microscope Basics Optical Sectioning Fluorescent Proteins Spinning Disk Superresolution
mTagBFP2 Fused to Human PCNA

mTagBFP2 Fused to Human PCNA

Blue fluorescent proteins are notorious for being very dim and having low photostability. Newly developed coral derivatives emitting in the blue region may improve multicolor imaging using fluorescent proteins that require blue as one of the complementary colors. mTagBFP and the improved mTagBFP2 are bright and photostable coral derivatives that may serve as useful blue markers in live-cell imaging. The cell culture presented in the digital image above is shown expressing a mEmerald fusion tag with proliferating cell nuclear antigen (PCNA), highlighting replication centers in the nuclei of cells in early G1 and S phase of the cell cycle. PCNA, which is a cofactor of DNA polymerase delta, is important for the synthesis of DNA and its repair.