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Rabbit Kidney Cells with mEmerald-Plastin

Plastin is a substance associated with proteins, phosphoric acids, and nucleic acids in cell nuclei, and is considered by some as the fundamental material of the nucleus. T- and L-plastin are very similar actin-bundling proteins involved in the regulation of bacterial invasion, cell morphology, lamellipodium protrusion, and tumor progression. T-plastin localizes mainly to the cytoplasm, whereas L-plastin distributes between the cytoplasm and nucleus in HeLa or Cos cells. Rabbit kidney epithelial cells (RK-13 line) appearing in the featured digital video sequence are expressing mEmerald fused to plastin.

The green fluorescent protein mEmerald has a peak excitation wavelength of 487 nanometers and peak emission wavelength of 509 nanometers. mEmerald is one of many variants of the enhanced Aequorea derivative EGFP. The brightness and photostability of mEmerald are both greater than that of their parent protein. mEmerald does exhibit a fast photobleaching component that can, however, raise difficulties in certain applications.