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Human Osteosarcoma cells with mRuby-Light Chain Myosin

Mysoins are an important group of molecular motor proteins present in nearly all eukaryotic cells. The main characteristics held in common by the various known myosin isoforms include actin-binding, force transducing, and ATP hydrolyzing capabilities. Most myosins are comprised of three domains: head, neck, and tail. The neck domain is the region that often serves as a binding site for light chain myosins, which are distinct proteins that form part of a macromolecular complex with other light chain and heavy chain myosins. Light chain myosins often function in a regulatory capacity.

Cultured human osteosarcoma cells (U-2 line) appear in this digital video sequence expressing a red fluorescent protein tag fused to light chain myosin. mRuby is a derivative of eqFP611, a far-red fluorescent protein derived from the bubble-tip anemone (Entacmaea quadricolor). Excitation and emission of mRuby peak at 558 and 605 nanometers, respectively.