Most information about the nature of living cells is discovered through light microscope observation. The image seen through a microscope is not a magnified point-by-point representation of the object, but is an abstract representation of the object that must be interpreted. This interpretation is influenced not only by the actual structure of the object, but the physical characteristics of light and the microscope components, the preparation of the sample, and the microscopist's background. In order to interpret the image correctly and fully, the microscopist must understand the interaction of light with the sample.
The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells which influence interpretation.