Rizzuto, R., Carrington, W., and Tuft, R. A.
Digital imaging microscopy in living cells.  Trends in Cell Biology 8: 288-292 (1998).

Fluorescence microscopy has undergone a resurgence in interest following the discovery of green-fluorescent protein (GFP) and its increasing use in live-cell imaging. This article describes an enhanced form of epifluorescence microscopy, digital imaging microscopy, that can be used to produce high-resolution three-dimensional images of samples labelled with GFP, or other fluorochromes, using simple instrumentation and image-restoration software.