Nienhaus, G. U., Nienhaus, K., Hölzle, A., Ivanchenko, S., Renzi, F., Oswald, F., Wolff, M., Schmitt, F., Röcker, C., Vallone, B., Weidenmann, W., Heilker, R., Nar, H., and Wiedenmann, J.
Photoconvertible fluorescent protein EosFP: Biophysical properties and cell biology applications.  Photochemistry and Photobiology 82: 351-358 (2006).

EosFP is a fluorescent protein from the coral Lobophyllia hemprichii that changes its fluorescence emission from green to red upon irradiation with near-UV light. Here we present the spectroscopic properties of wild-type EosFP and a variety of monomeric and dimeric mutants and provide a structural interpretation of its oligomerization and photoconversion, which is based on X-ray structure analysis of the green and red species that we reported recently. Because functional expression of the monomeric EosFP variant is limited to temperatures of 308C, we have developed a tandem dimer. This construct, in which two EosFP subunits are connected by a flexible 12 amino acid linker, expresses well after fusion with the androgen and endothelin A receptors at 37C. A variety of applications in cellular imaging, developmental biology and automated highcontent screening applications are presented, which demonstrate that EosFP is a powerful tool for in vivo monitoring of cellular processes.