Coling, D. and Kachar, B.
Principles and Application of Fluorescence Microscopy.  Current Protocols in Molecular Biology: 14.10.1-14.10.44 (2003).

This overview discusses the principle of fluorescence along with practical discussions of fluorescent molecular probes, filters and filter sets, multiband filters and multi-dye fluorescence, light sources, objective lenses, image resolution and the point-spread function, fluorescence microscopy of living cells, and immunolabeling.