Michalet, X., Lacoste, T. D. and Weiss, S.
Ultrahigh-resolution colocalization of spectrally separable point-like fluorescent probes. Methods 25: 87-102 (2001).

An ultrahigh-resolution colocalization method based on the simultaneous acquisition and analysis of spectrally separated images of the excitation point-spread function of point-like fluorescent probes is reviewed. It is shown that molecular distances can be measured with accuracy better than 10 nm using conventional far-field optics. A detailed account of the methodology, theoretical considerations, signal processing, and data fitting algorithms is given.