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Embryonic Rat Thoracic Aorta Smooth Muscle Fibroblast Cells

Peroxisomal membrane protein 70 (PMP 70) is generally classified as a member of the half ATP-binding cassette (ABC) transporter superfamily of proteins. The constituents of this superfamily all contain ATP-binding folds and are thought to be associated with transport across membranes. Specifically, PMP 70 is believed to function in the metabolic transport of long chain acyl-CoA across peroxisomal membranes.

Embryonic Rat Thoracic Aorta
Smooth Muscle Fibroblast Cells

The peroxisomes present in the rat thoracic aorta cell culture illustrated in the digital image above were immunofluorescently labeled with Alexa Fluor 647 (pseudocolored yellow) conjugated to goat secondary antibody fragments directed against rabbit primary antibodies to peroxisomal membrane protein 70 (PMP 70). The culture was also labeled with Alexa Fluor 488 conjugated to phalloidin, MitoTracker Red CMXRos, and Hoechst 33258, in order to target F-actin, mitochondria, and nuclear DNA, respectively. Images were recorded in grayscale with a Hamamatsu ORCA AG camera system coupled to a ZEISS Axio Imager microscope equipped with bandpass emission fluorescence filter optical blocks provided by Chroma and Semrock. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.