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Bovine Pulmonary Artery Epithelial Cells

The BPAE cell line was initiated in January 1979 by P. Del Vecchio from the main stem of a pulmonary artery belonging to a young cow (Bos taurus). Pulmonary arteries, which extend from the heart to the lungs, are the only arteries in the mammalian body that carry dark, unoxygenated blood.

Bovine Pulmonary Artery Endothelial Cells

The culture of bovine pulmonary artery endothelial cells appearing in the digital image above was stained with MitoTracker Red CMXRos, which highlights the extensive intracellular mitochondrial network. The specimen was also labeled for filamentous actin and DNA with Alexa Fluor 488 (green emission) conjugated to phalloidin and DAPI (blue emission), respectively. Images were recorded in grayscale with a Hamamatsu ORCA AG camera system coupled to a ZEISS Axio Imager microscope equipped with bandpass emission fluorescence filter optical blocks provided by Chroma and Semrock. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.