Contact Us | Carl Zeiss

Zeiss Logo

Education in Microscopy and Digital Imaging

ZEISS Microscopy ¦ Products ¦ Solutions ¦ Support ¦ Online Shop ¦ ZEISS International

Light Sources Spectral Imaging Wavelength Selection Microscope Basics Optical Sectioning Fluorescent Proteins Spinning Disk Superresolution
Bovine Pulmonary Artery Endothelial Cells

An important membrane polypeptide in peroxisomes, the peroxisomal membrane protein 70 (PMP 70) is generally considered to be one of the half ATP-binding cassette (ABC) transporter proteins. Members of this superfamily contain ATP-binding folds and are thought to be commonly involved in transport across membranes.

Bovine Pulmonary Artery Endothelial Cells

The peroxisomes present in the bovine pulmonary artery cell culture illustrated in the digital image above were immunofluorescently labeled with Alexa Fluor 633 conjugated to goat secondary antibody fragments directed against rabbit primary antibodies to PMP 70. In addition, the culture was also labeled for F-actin with Alexa Fluor 488 conjugated to phalloidin and for the intracellular mitochondrial network with MitoTracker Red CMXRos. Nuclei were counterstained with Hoechst 33258. Images were recorded in grayscale with a Hamamatsu ORCA AG camera system coupled to a ZEISS Axio Imager microscope equipped with bandpass emission fluorescence filter optical blocks provided by Chroma and Semrock. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.