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Grey Fox Lung Fibroblast Cells

The FoLu cell line was established from the lung tissue of an adult female gray fox (Urocyon cineroargenteus). FoLu cells exhibit many of the typical characteristics associated with fibroblasts and testing indicates that the line is susceptible to vesicular stomatitis, herpes simplex, and vaccinia viruses.

Grey Fox Lung Fibroblast Cells

MitoTracker Red CMXRos is a derivative of X-rosamine that selectively binds with mitochondria. By treating a culture of FoLu cells (shown above) with the MitoTracker probe, details of the mitochondrial network were visualized. The culture was also immunofluorescently labeled with Alexa Fluor 647 conjugated to goat secondary antibody fragments directed against rabbit primary antibodies to PMP 70 in order to target peroxisomes and stained with Hoechst 33258 to image cell nuclei. Images were recorded in grayscale with a Hamamatsu ORCA AG camera system coupled to a ZEISS Axio Imager microscope equipped with bandpass emission fluorescence filter optical blocks provided by Chroma and Semrock. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.