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Welcome

The Carl Zeiss MicroImaging Online Campus website explores the fascinating world of optical microscopy and provides the necessary background to understand both the basic concepts and advanced principles.

Emission Fingerprinting - NEW!

Learn how lambda stacks are used in spectral imaging to generate emission fingerprints.

Superresolution Imaging

Examine the basic principles of photoactivated localization microscopy (PALM) imaging.

LED Illumination

Explore the use of LEDs for rapid wavelength switching in fluorescence microscopy.

Colocalization Analysis

Colocalization is a powerful tool for analyzing the spatial overlap of fluorescent probes.

Fluorescent Proteins

Download the latest review articles on fluorescent protein technology.

Spinning Disk Confocal Microscopy

Spinning disk confocal microscopy is excellent for investigation of dynamics in living cells.

Latest Review Articles

Introduction to Spectral Imaging and Linear Unmixing

Spectral imaging coupled to linear unmixing is becoming an important staple in the microscopist's toolbox, particularly when applied to the elimination of autofluorescence and for FRET investigations.

Introduction to Spinning Disk Microscopy

Review spinning disk microscopy from a historical perspective, as well as current instrumentation that is being used today. Also included is information on resolution and digital camera systems.

Light-Emitting Diodes (LEDs)

Among the most promising of emerging technologies for illumination in optical microscopy is the light-emitting diode (LED).

Introduction to Fluorescent Proteins

Fluorescent protein development strategies are centered on fine-tuning existing variants and developing new proteins emitting in the red wavelengths.