The in vitro culture of whole tissues and isolated cells was first undertaken in the early 1900s as a technique for investigating the behavior of normal animal cells in an isolated and highly controlled environment. The term tissue culture arose because most of the early cell lines were derived from primary tissue explants, a technique that dominated the field for over 50 years. As established cell lines that can be subcultured and preserved by freezing have slowly emerged, the application of well-defined normal and transformed cells in a host of biomedical investigations has become an important staple in the advanced development of cellular and molecular biology. This fluorescence image gallery explores a number of cell lines labeled with a variety of fluorophores using both traditional staining methods as well as immunofluorescence techniques.
- Bovine Pulmonary Artery Cells 1
3-Color Fluorescence - Bovine Pulmonary Artery Cells 2
3-Color Fluorescence - Bovine Pulmonary Artery Cells 3
4-Color Fluorescence - Bovine Pulmonary Artery Cells 4
3-Color Fluorescence - Madin-Darby Canine Kidney Cells
2-Color Fluorescence - Human Cervical Adenocarcinoma (HeLa)
2-Color Fluorescence - Indian Muntjac Deer Skin Fibroblasts 1
3-Color Fluorescence - Indian Muntjac Deer Skin Fibroblasts 2
3-Color Fluorescence - Indian Muntjac Deer Skin Fibroblasts 3
3-Color Fluorescence - Indian Muntjac Deer Skin Fibroblasts 4
3-Color Fluorescence - Gray Fox Lung Fibroblasts 1
3-Color Fluorescence - Gray Fox Lung Fibroblasts 2
3-Color Fluorescence - Gray Fox Lung Fibroblasts 3
3-Color Fluorescence - Gray Fox Lung Fibroblasts 4
3-Color Fluorescence - Horse Skin Fibroblast Cells 1
3-Color Fluorescence - Horse Skin Fibroblast Cells 2
3-Color Fluorescence - Human Fetal Lung Fibroblasts 1
4-Color Fluorescence - Human Fetal Lung Fibroblasts 2
4-Color Fluorescence - Human Fetal Lung Fibroblasts 3
4-Color Fluorescence - Human Fetal Lung Fibroblasts 4
3-Color Fluorescence - Human Lung Carcinoma Epithelial Cells
3-Color Fluorescence - Mink Uterus Epithelial Cells
3-Color Fluorescence - Transformed Monkey Kidney Cells 1
3-Color Fluorescence - Transformed Monkey Kidney Cells 2
3-Color Fluorescence - Normal Monkey Kidney Cells
3-Color Fluorescence - Owl Monkey Kidney Cells 1
2-Color Fluorescence - Owl Monkey Kidney Cells 2
3-Color Fluorescence - Mouse Embryo Fibroblast Cells
2-Color Fluorescence - Opossum Kidney Epithelial Cells
3-Color Fluorescence - Ovine Kidney Epithelial Cells
3-Color Fluorescence - Raccoon Uterus Fibroblasts 1
2-Color Fluorescence - Raccoon Uterus Fibroblasts 2
3-Color Fluorescence - Rat Kangaroo Kidney Cells
3-Color Fluorescence - Rat Thoracic Aorta Fibroblasts 1
3-Color Fluorescence - Rat Thoracic Aorta Fibroblasts 2
2-Color Fluorescence - Rat Thoracic Aorta Fibroblasts 3
3-Color Fluorescence - Rat Thoracic Aorta Fibroblasts 4
3-Color Fluorescence - Rat Thoracic Aorta Fibroblasts 5
4-Color Fluorescence - Rat Thoracic Aorta Fibroblasts 6
4-Color Fluorescence - Rat Thoracic Aorta Fibroblasts 7
3-Color Fluorescence - Rat Thoracic Aorta Fibroblasts 8
3-Color Fluorescence - Rat Thoracic Aorta Fibroblasts 9
3-Color Fluorescence - Rat Thoracic Aorta Fibroblasts 10
3-Color Fluorescence - Rat Thoracic Aorta Fibroblasts 11
2-Color Fluorescence - Tahr Ovary Fibroblast Cells
3-Color Fluorescence
The digital images in this gallery explore multiple staining using a variety of cell lines labeled with fluorophores that target specific organelles. Images were produced using a ZEISS AxioImager upright microscope equipped with filter sets for ultraviolet, cyan, green, and yellow excitation with bandpass emission.