Imaging through thick tissue sections is hampered by blurring and stray light that occurs due to fluorescence emission arising from regions that are removed from the objective focal plane. In many cases, the effects of this high background signal can be so severe that finely labeled structures and critical image details are completely obscured. The ZEISS ApoTome employs structured illumination to generate optical sections of thick specimens that exhibit dramatically increased sharpness and contrast. These optical sections are useful to create three-dimensional (3D) reconstructions of the original sample. The digital images in this gallery are reconstructions of animal and plant tissue optical sections acquired with the ApoTome coupled to a metal halide light source.
- Buttercup Root
Image 1 - 40x (Autofluorescence) - Lily Embryo
Image 1 - 40x (Autofluorescence) - Lily Flower Bud
Image 1 - 40x (Autofluorescence) - Mixed Pollen
Image 1 - 63x - Mouse Bladder Tissue
Image 1 - 40x - Mouse Bladder Tissue
Image 2 - 40x - Mouse Brain Tissue
Image 1 - 20µm - 40x - Mouse Brain Tissue
Image 2 - 20µm - 40x - Mouse Duodenum Tissue
Image 1 - 40x - Mouse Duodenum Tissue
Image 2 - 40x - Mouse Duodenum Tissue
Image 3 - 40x - Mouse Ovary Tissue
Image 1 - 40x - Mouse Ovary Tissue
Image 2 - 40x - Mouse Prostate Tissue
Image 1 - 40x - Mouse Prostate Tissue
Image 2 - 40x - Mouse Prostate Tissue
Image 3 - 40x - Mouse Skin Tissue
Image 1 - 63x - Mouse Skin Tissue
Image 2 - 63x - Mouse Smooth Muscle Tissue
Image 1 - 40x - Mouse Smooth Muscle Tissue
Image 2 - 40x - Mouse Smooth Muscle Tissue
Image 3 - 40x - Mouse Stomach Fundus Tissue
Image 1 - 40x - Mouse Stomach Fundus Tissue
Image 2 - 40x - Mouse Stomach Fundus Tissue
Image 3 - 40x - Mouse Tongue Tissue
Image 1 - 40x - Mouse Tongue Tissue
Image 2 - 40x - Mouse Vagina Tissue
Image 1 - 40x - Mouse Vas Deferens Tissue
Image 1 - 40x - Mouse Vas Deferens Tissue
Image 2 - 40x - Sclerenchyma Tissue
Image 1 - 40x (Autofluorescence)